Purification and Identification of Horse Serum IgA

Residue of horse serum purification as a nitrogen source for growth of microorganisms, l-Clostridia

Purification of horse serum cholinesterase by preparative polyacrylamide gel electrophoresis.

Preparative polyacrylamide gel electrophoresis has been used in the final stages of purification to prepare horse serum cholinesterase which was at least 9.5% pure, as judged by analytical polyacrylamide gel electrophoresis. The starting material, already purified about 109-fold by the Strelitz method, was purified an additional 89-fold by the procedure. The method involved Sephadex G-ZOO and S...

Serum anti-rabbit and anti-horse IgG, IgA, and IgM in kidney transplant recipients.

BACKGROUND The therapeutic efficacy of horse antilymphocyte globulins (ALG) or of rabbit antithymocyte globulins (ATG), used for both the prevention and treatment of allograft rejection has been well documented. However, clinical use of these heterologous antibodies can result in the production of antibodies against horse or rabbit proteins and in the development of serum sickness via circulati...

Serum IgM and IgA

SYNOPSIS Immunofluorescent serum IgM and/or a significant level of IgA antibody was detected in 87 % of 39 cases of current or recent influenza A infection from two to 84 days after the onset of illness. Secondary IgM staining occurred in 5% of sera and a significant correlation was found between complement-fixing and class-specific antibodies. It was estimated that the immunofluorescent test c...

Purification, identification and characterisation of seprase from bovine serum.

The study and identification for the first time of a soluble form of a seprase activity from bovine serum is presented. To date, this activity has only been reported to be an integral membrane protease but has been known to shed from its membrane. The activity was purified 30,197-fold to homogeneity, using a combination of column chromatographies, from bovine serum. Inhibition by DFP, resulting...